Research – Blainey Lab

Tags

Top Highlights

• Pooled optical genetic screening in human cells

• ngle cell genomic and functional analysis, drug screening, and genomic screening by combining diverse molecular, optical, and microfluidic technologies.

• Scalable combinatorial screening

• Accurate and sensitive measurement of somatic mutation flow across cells

• Robust and accurate systems for single-cell genomics at scale

• d in situ sequencing

• This capability reduces the cost for spatially and temporally-resolved screens by multiple orders of magnitude and extends large-scale pooled screening into new biological models and disease areas.

• robustness, compartmentalization of small-molecule solutes, and integration with imaging that enabled the first large-scale application of micro-droplets for drug screening.

• synergize to re-activate antibiotic drugs against Gram-negative bacterial pathogen

• The group developed one method based on a new microfluidics concept we call virtual microfluidics that eliminates the dependence on specialized equipment and a molecular enrichment method that makes a 100-fold improvement in sensitivity/throughput for rare target cells in pooled single-cell RNA-seq libraries

• My group developed a concept to jointly analyze variants across samples related by a lineage prior (rather than one at a time) to improve the sensitivity and specificity of somatic mutation detection and resolve such events in individual related cells.