observe here whether the samples from the case group (CASE) and samples from the control group (CTRL) can be split into two distinct clusters
improve the quality of the input reads.
against the null hypothesis
activity of the gene stays the same in two different condition
uared coefficient of variation
An MA plot is useful to observe if the data normalization worked wel
x-axis denotes the average of normalized counts
the log fold change i
horizontal 0 line
genes are not
check the biological reproducibility of the sample replicates
A design formula
ead count table
a subset of the count table
op 100 most variable genes
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