Overexpression of the Chitosanase Gene in Fusarium solani via Agrobacterium tumefaciens-Mediated Transformation - Current Microbiology
link.springer.com/article/10.1007/s00284-008-9334-2
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Southern blots indicated that all five transformants contained a single copy of the T-DNA integration
herbicide-resistance gene bar as the selection marker in F. solani transformation, growth inhibition of the herbicide phosphinothricin (PPT) on F. solani 0114 was tested.
chitosanase gene (csn)
pCAMBIA 1300 was constructed.
under control of the Aspergillus nidulans gpdA promoter and A. nidulans trpC terminator
Agrobacterium tumefaciens-mediated transformation, and the herbicide-resistance gene bar from Streptomyces hygroscopicus was used as the selection marker
significant increase in chitosanase production (~2.1-fold than control) were obtained.
12 resistant colonies were obtained.
5 of the 12 colonies showed a significant increase in chitosanase production
genomic DNA from all five colonies was extracted and digested with EcoRI and BamHI.
so multiple hybridizing bands would be indicative of multiple T-DNA copies.
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