The phosphorus deficiency was maintained for 1 week, after which another 3 L phosphorus-deficient nutrient solution was applied and drained away freely to remove accumulated strigolactones from the rhizosphere.
Strigolactones were also measured in root extracts of the first four highest strigolactone producing and last four lowest producing rice cultivars.
The relationship between various strigolactones and S. hermonthica germination and tillering was analysed by correlation analysis using R.
the average strigolactone peak areas of the rice cultivars were symmetrically scaled by the standard deviation (SD) of the samples and the individual strigolactones. The SD-scaled data were used (without log transformation) in redundancy analysis (RDA), to examine whether the variation in the strigolactones explains the variation in average infection parameters of the rice genotypes.
Bars represent means of peak areas of the individual strigolactones as determined by multiple reaction monitoring liquid chromatography–mass spectrometry in triplicate.
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