They were imbibed in H2O or 20 mm H2O2 for 24 h, washed twice with H2O and placed in Petri dishes with two layers of filter paper moistened with H2O.
Protein carbonylation is considered as a potent biomarker of oxidative stress, which can play regulatory roles, such as seed dormancy alleviation (El-Maarouf Bouteau et al. 2007) and salt-stress tolerance in leaves (Tanou et al. 2009).
20 mm H2O2
oxidative features such as protein carbonylation, endogenous H2O2 and lipid peroxidation levels
H2O2 treatment of the pea seeds increased their endogenous H2O2 content and caused carbonylation of storage proteins and of several metabolic enzymes
expression of one of them, PsMAPK2, largely increased upon pea seed imbibition in H2O2, whereas no change could be observed in expression of the other, PsMAPK3
levels of several phytohormones such as 1-aminocyclopropane carboxylic acid, indole-3-acetic acid and zeatin appeared to correlate with the measured oxidative indicators and with the expression of PsMAPK2.
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