ability of the bacterial cell wall to retain the crystal violet dye during solvent treatment
Gram-positive microorganisms have higher peptidoglycan conten
gram-negative organisms have higher lipid content.
with the use of solvent,
the lipid layer from gram-negative organisms is dissolved
the dissolution of the lipid layer, gram negatives lose the primary stain
solvent dehydrates the gram-positive cell walls with the closure of pores preventing diffusion of violet-iodine complex, and thus, bacteria remain stained.
n to give decolorized gram-negative bacteria pink color for easier identification.
known as counterstain.
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