Gram Staining - PubMed

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• ability of the bacterial cell wall to retain the crystal violet dye during solvent treatment

• Gram-positive microorganisms have higher peptidoglycan conten

• gram-negative organisms have higher lipid content.

• with the use of solvent,

• the lipid layer from gram-negative organisms is dissolved

• the dissolution of the lipid layer, gram negatives lose the primary stain

• solvent dehydrates the gram-positive cell walls with the closure of pores preventing diffusion of violet-iodine complex, and thus, bacteria remain stained.

• n to give decolorized gram-negative bacteria pink color for easier identification.

• known as counterstain.