RNA sequencing data analysis using R and the Artemis HPC: Building a genome index
sydney-informatics-hub.github.io/training-RNAseq/02-BuildAGenomeIndex/index.html
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STAR to index the genome fasta file
“splicing-aware” aligners
align RNA-seq data
natural “gaps”
as a result of splicing.
construct and index the genome
de novo transcripome assembly
genome sequence
annotation file
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