Laboratory Diagnosis of Candidiasis

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• easy and rapid commercialized latex agglutination test, Bichro-Dubli Fumouze

• Oral swab is collected, followed by microscopy and culture on selective media

• non-specific

• Swab culture is normally the first test that is commonly done for identification of Candida species causing Candidiasis

• It takes 1 to 3 days to have results

• differentiate C. albicans from C. dubliniensis by detecting specific antigens located on the surface of C. dubliniensis blastoconidia

• API and VITEK (

• developed for Candida species identification.

• PI 20C system

• first available commercial kits used for the identification of yeas

• ID 32C system (bioMérieux, France)

• 12 substrates more than API which can enable identification of a diverse set of clinically important yeasts and can also differentiate between C. albicans and C. dubliniensis

• e Vitek 2 system is able to identify and detect Candida species and their antifungal susceptibility profile

• BDG Fungitell assay

• high false-positive, low sensitivity and specificity results

• screening purposes

• Molecular methods are more accurate and rapid in detecting Candida species.

• apidly detect both primary and secondary antifungal resistance alleles

• Polymerase chain reaction-based techniques

• fast and easy use