were coupled with
2.1. Conventional APTES-GA and EDC-NHS Coating Techniques
hydrolysis of ethoxy (–C 2H 5) groups from APTES, leading to the formation of silanols (Si–OH)
both aldehydes of some GA molecules may bind with APTES,
This produced Caro’s acid (H2SO5), which can cause dissociation and generate a hydroxyl radical (OH*) to produce hydroxyl groups (–OH) by a hydroxylation process
the hydroxylated silicon surface was immersed in APTES solution (2% APTES in 95% ethanol) for 1 h at 68 °C
Each protein was conjugated with 0.7 mg/mL of EDC and 0.6 mg/mL of NHS in the activation buffer (0.1 M MES, 0.5 M NaCl, pH 6.0) for 15 min at room temperature (premixing)
Element composition after each step of GOx coating using (a) APTES-GA technique and (b) APTES-(EDC/NHS) technique.
Glasp is a social web highlighter that people can highlight and organize quotes and thoughts from the web, and access other like-minded people’s learning.