mScarlet is currently our first-choice red fluorescent protein for visualizing gene expression in vivo (Bindels et al. 2017). Compared to other RFPs like mCherry or mKate2, mScarlet is less prone to unwanted oligomerization and yields superior brightness and photostability (Bindels et al. 2017).
Despite the improved performance of contemporary monomeric fluorophores like mScarlet and mNeonGreen (Heppert et al. 2016; Bindels et al. 2017), sometimes the relatively large size of these tags can disrupt the function of the targeted protein.
As with other SEC plasmids (Dickinson et al. 2015), an in-frame epitope tag is included (in this case 3xMyc) to allow purification or immunostaining of the tiny-tagged target protein
N- or C-terminal tagging of any protein of interest
dopaminergic PDE neurons
SEC-based CRISPR/Cas9 (Dickinson et al. 2015) was used to insert all constructs into the genome.
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