B-lymphocytes contains HPRT1 gene which codes for enzyme Hypoxanthine-guanine phosphoribosyltransferase (HGPRT).
Monoclonal antibodies are produced by hybridoma technology.
specificity against specific antigen, it is known as monoclonal antibody.
monoclonal antibodies are derived from a single clone of cell which recognize only one kind of antigen
fusion of following two types of cells-a lymphocytes and tumor cell.
Ability to produce large amount of pure antibodies as lymphocytes and ability to grow or multiply indefinitely like tumor cell.
usion is done by using Polyethylene glycol (PEG)
B-cells is extracted from spleen
myeloma cell from bone marrow.
formation of hybridoma cells formed by fusion of B-cell and myeloma cell.
synthesis of nucleotides from Hypoxanthine present in culture medium.
B- cells can grow in medium containing Hypoxanthine amonopterin thymine (HAT media).
myeloma cell lack HPRT1
does not grow in HAT medium.
myeloma cell or those do not fused at all die in HAT medium since they do not utilize Hypoxanthine.
only hybridoma cell ie. fused cell between myeloma and B-cell can survive and divide in HAT medium.
Screening is done to select hybridoma cells which are the desired cell for monoclonal antibodies production.
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