The joint knockdown of TPRX1/2/L [TPRX triple KD (TKD)] led to severe defects in development and ZGA. About 31% of ZGA genes that preferentially contain PRD-like TF-binding motifs at promoters and nearby putative enhancers were down-regulated in TPRX TKD embryos.
TPRX transcription factor family, which includes TPRXL, a maternally inherited factor, and TPRX1/2, two early transcribed transcription factors after fertilization,
ZGA marks the first transcription event in a new life and the onset of the embryonic program.
key ZGA transcription factors (TFs) have been well characterized in other species such as zebrafish and fly,
jointly profiled the translatome and transcriptome across eight stages of human oocytes and early embryos
a group of PRD-like homeobox TFs became highly translated before or during ZGA
ectopically expressed TPRXs could bind and activate a subset of ZGA genes in hESCs.