Translatome and transcriptome co-profiling reveals a role of TPRXs in human zygotic genome activation
www.science.org/doi/10.1126/science.abo7923
1 Users
0 Comments
8 Highlights
0 Notes
Tags
Top Highlights
The joint knockdown of TPRX1/2/L [TPRX triple KD (TKD)] led to severe defects in development and ZGA. About 31% of ZGA genes that preferentially contain PRD-like TF-binding motifs at promoters and nearby putative enhancers were down-regulated in TPRX TKD embryos.
TPRX transcription factor family, which includes TPRXL, a maternally inherited factor, and TPRX1/2, two early transcribed transcription factors after fertilization,
ZGA marks the first transcription event in a new life and the onset of the embryonic program.
key ZGA transcription factors (TFs) have been well characterized in other species such as zebrafish and fly,
Ribo-lite
jointly profiled the translatome and transcriptome across eight stages of human oocytes and early embryos
a group of PRD-like homeobox TFs became highly translated before or during ZGA
ectopically expressed TPRXs could bind and activate a subset of ZGA genes in hESCs.
Ready to highlight and find good content?
Glasp is a social web highlighter that people can highlight and organize quotes and thoughts from the web, and access other like-minded people’s learning.